Education Secretary Linda McMahon issued a warning Tuesday to organizations and institutions continuing to allow transgender athletes to compete in women’s and girls’ sports, directly referencing those not complying with President Donald Trump’s executive order signed in February, as reported by Fox News.

The order, titled “No Men in Women’s Sports,” prohibits biological males from competing in female athletic competitions. Despite its enactment, several athletic associations and governing bodies have not complied, prompting further action from the Trump administration.

Mar 18, 2022; Atlanta, Georgia, USA; Penn Quakers swimmer Lia Thomas holds a trophy after finishing fifth in the 200 free at the NCAA Swimming & Diving Championships as Kentucky Wildcats swimmer Riley Gaines looks on at Georgia Tech. Mandatory Credit: Brett Davis-USA TODAY Sports

“To all the entities that continue to allow men to compete in women’s sports and use women’s intimate facilities: there’s a new sheriff in town,” McMahon posted on X. “The Trump Administration will not allow you to get away with denying women’s civil rights any longer.”

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Several high school athletic associations have not adhered to the executive order, but the most significant pushback has come from the state of Maine.

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Since February, tensions between the Trump administration and Maine officials have escalated.

Fourteen Maine state senators sent a letter Tuesday to the White House condemning Governor Janet Mills for continuing to support policies that enable transgender inclusion in women’s sports.

Maine Governor Janet Mills joined state and local public safety officials in Lewiston in a news conference Thursday morning in the wake of two shootings. At least a dozen people were killed in the area on Oct. 25, 2023.

The letter stated that Maine’s approach violates Title IX and the Family Educational Rights and Privacy Act. The U.S. Department of Agriculture (USDA) froze federal funding to the state last week, citing noncompliance. A referral to the Department of Justice has also been made.

On Monday, Maine officials filed a lawsuit against the USDA in response to the funding freeze. President Trump publicly addressed the matter, promising legal action against Governor Mills.

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The dispute has widened beyond Maine. USA Fencing has come under fire after a biological male competed in a women’s division, prompting fencer Stephanie Turner to kneel in protest. Turner expressed her objection to being forced to compete against a male opponent.

Nov 6, 2023; Boston, MA, USA; Schuyler Bailar poses for a portrait in a suit that reads “trans athlete” at Harvard’s Blodgett Pool. Bailar is an author, educator and swimmer who built a popular social media presence for being outspoken and squashing misinformation about trans people in sports. He swam for the Harvard Men’s Swimming and Diving team as the first openly transgender NCAA Division I swimmer. Mandatory Credit: Jasper Colt-USA TODAY NETWORK

Disc golf player Abigail Wilson also raised concerns, publicly declaring the situation unfair. She criticized the allowance of transgender athletes in female sports, arguing that it compromises the integrity of competition.

The NCAA has also drawn criticism after a transgender athlete competed for Ithaca College during the Cayuga Duals.

Although the athlete only participated in the third varsity eight event—which does not count toward NCAA championship qualification—Ithaca College defended the situation as a “misunderstanding.”

Apr 20, 2024; Fort Worth, TX, USA; LSU Tigers gymnast Olivia Dunne kisses the trophy after the LSU Tigers gymnastics team wins the national championship in the 2024 Womens National Gymnastics Championship at Dickies Arena. Mandatory Credit: Jerome Miron-USA TODAY Sports

Women’s rowing was recently added to the list of sports facing scrutiny, with questions raised over how NCAA policy changes may allow further exceptions.

The Trump administration has vowed to continue enforcement of the executive order and hold accountable those institutions failing to comply.